Western Analytical Products Datasheets for HPLC Columns

High performance liquid chromatography (HPLC) columns use a liquid mobile phase to separate the components of a mixture.
HPLC Columns: Learn more

Product Name Notes
"0.5-10 ml Samples, Syringe Mounted"
"1-10 µl Samples, Pipette mounted tip (Embedded tip)"
"1-10 µl Samples, Pipette mounted tip (Packed Tip)"
"10-200 µl Samples, Pipette Mounted Tip (Embedded Tip)"
"10-200 µl Samples, Pipette Mounted Tip (Packed Tip)"
"100 µm ID x 1/32"" OD, 100 mm long, pack of 2"
"100 µm ID x 1/32"" OD, 150 mm long, pack of 2"
"100 µm ID x 1/32"" OD, 50 mm long, pack of 2"
"150 µm ID x 1/32"" OD, 100 mm long, pack of 2"
"150 µm ID x 1/32"" OD, 150 mm long, pack of 2"
"150 µm ID x 1/32"" OD, 50 mm long, pack of 2"
"25 µm ID x 1/32"" OD, 100 mm long, pack of 2"
"25 µm ID x 1/32"" OD, 150 mm long, pack of 2"
"25 µm ID x 1/32"" OD, 50 mm long, pack of 2"
"50 µm ID x 1/32"" OD, 100 mm long, pack of 2"
"50 µm ID x 1/32"" OD, 150 mm long, pack of 2"
"50 µm ID x 1/32"" OD, 50 mm long, pack of 2"
"75 µm ID x 1/32"" OD, 100 mm long, pack of 2"
"75 µm ID x 1/32"" OD, 150 mm long, pack of 2"
"75 µm ID x 1/32"" OD, 50 mm long, pack of 2"
" Length "
" Particle Size "
" Phase "
" Pore Size "
" Optimized for Protein and Peptide separation/Purificat ion Nonconductive and conductive options Packed and Unpacked columns Upchurch scientific capillary sample Trap columns are ideal for separating and concentrating and/or purifying...
"1.0 mm ID x 2 cm Ideal for Microbore HPLC Easily Dry Packed (or Slurry Packed with Adapter) Made of PEEK Polymer and Stainless Steel This Upchurch Scientific® ultralow...
"2.0 mm ID x 2 cm Easy to Pack Available as a Kit with Funnel and Extra Frits The C-130B is our most popular guard column. HPLC users find...
"Crude Synthetic Phosphopeptide: Numerous failure sequences may not be separated by reversed-phase, but the below chromatogram demonstrates the PolyCAT A, when using an acetic acid gradient, will yield a...
"For Cation-Exchange The PolyCAT A is made though a unique process for attaching Poly (aspartic acid) covalently to silica. Proteins elute from this polypeptide coating in sharp peaks with...
"HAISpeed20 guard cartridges provide unique Protection for 20 mm I.D. prep columns. ""Prep is a dirty business,"" and expensive columns can be quickly destroyed by crude samples if proper...
"No Tools Required for Leak-Free Operation Even though Higgins Analytical cartridge holders do not require any tools for assembly and disassembly, their leak-free operation assures that maximum performance will...
"Triptic Digest of HeLa Cell Nuclear Proteins PolySULFOETHYL A is capable of pulling the +2 peptides away from the +1 peptides. This makes it possible to collect and identify...
"BenchMark columns are designed to suit the majority of standard laboratory chromatography applications. They are ideal for aqueous systems and compatible with solvents used in common liquid chromatography applications such...
"For complete convenience, try the Upchurch Scientific guard column kit with packing material. This kit contains 10 replacement frits, a packing funnel and 3 g of C18 reversed phase."
"HiT columns are designed for use at elevated temperature. Borosilicate glass and PEEK construction gives stability at temperatures up to 150°C. Ideal for use as reactor columns for flow chemistry...
"Hydrophilic Interaction Liquid Chromatography (HILIC) is a variation of normal-phase chromatography which can be performed with partially aqueous mobile phases. This permits normal phase separation of peptides, carbohydrates, nucleic acids,...
"Most proteins have isoelectric points below 7, and are best purified or analyzed by anion-exchange chromatography. PolyWAX LP is a weak anion-exchange (WAX) material developed for chromatography of enzymes and...
"no Frit, PEEK"
"no Frit, SST"
"SolventPlus columns are designed for maximum chemical compatibility. Borosilicate glass and PTFE construction, with Chemraz® O-rings and PTFE frits, only the most inert materials are present in the fluid path.
"These materials separate proteins on the basis of hydrophobic character, as does Reversed-Phase Chromatography. HIC uses totally aqueous buffers, maintaining tertiary structure and biological activity. Typically, a sample is eluted...
"This strong cation exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7 - 3.0, peptides lose their (-) charges and have net (+) charge. They can...
"When performing SEC of peptides, one generally obtains the best correlation of retention times and molecular weights if the mobile phase is acidic and contains some organic solvent. Peaks: (1)...
100 µm ID x 2.5 cm x 360 µm OD
Black PEEK
Green PEEK
Natural PEEK
PEEK
PEGylated protein (20-30KD) showing alternate PEG sites clearly resolved. The minor peaks near the large peak are proteins where the PEG chain was attached to the lysine residues other than...
SST Frit
with SST Frits
with Ti Frits