The OD8-DCL659-D is a dichroic beamsplitter for fluorescence imaging, designed to reflect excitation light and transmit emission light for Cy5™ and similar fluorophores. It features a 50% cut-on edge at 659 nm, with reflection ≥ 98% avg over the excitation passband and transmission ≥ 96% avg over the emission passband. The filter has an optical density ≥ 10 avg blocking in the excitation and emission passbands, verified to OD8. It is mounted in a rectangular housing measuring 25.2 x 35.6 mm with a 1.0 mm fused silica substrate, supports an angle of incidence of 45° ± 1.5°, and has parallelism ≤ 5 arcseconds and transmitted wavefront error ≤ 1 wave PV @ 632.8 nm. Orientation is indicated by an arrow on the edge of the dichroic.
The OD8-DCL659-D Dichroic Beamsplitter is specified to reflect the excitation and transmit the emission of Cy5™ and supports similar alternative fluorophores, designed in conjunction with and . The filter is manufactured to provide maximum signal and sensitivity delivering exceptionally high excitation passband reflection ≥ 98% avg and emission passband transmission ≥ 96% avg for a 659 nm 50% Cut-on edge wavelength.
Alternate Fluorophores
In addition to Cy5™ the following fluorophores are also compatible with this set:
Abberior STAR 635, Abberior STAR RED, Alexa Fluor® 647, Alexa Fluor® 660, APC, ATTO 635, ATTO 647, ATTO 655, BODIPY® 650/665-X, C5-Thiacyanine, Carboxynapthofluores
Optimized Coatings and Engineering Expertise
Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance. Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries.
Environmental Stability and Physical Durability
The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.
| Newport MKS | |
|---|---|
| Product Category | Polarizing Cube Beamsplitters |
| Product Number | OD8-DCL659-D |
| Product Name | ODiate® Dichroic Beamsplitter, 25.2x35.6 mm, 659 nm 50% Cut-on Edge Wavelength |
| Side Length | 35.6 mm (1.4 inch) |