The Hamilton PRP-C18 HPLC column, part number 79680, is a 2.1 x 150 mm column with 5 µm particle size and PEEK construction, designed for high-efficiency reversed-phase separations across a wide pH range (1-13) and temperatures up to 100°C. It features a rigid, inert stationary phase that resists stripping, bleeding, or dissolution under extreme conditions, enabling reproducible performance and extended column life. The column supports rapid separations with sharp peaks under fast gradients and elevated flow rates, and maintains efficiency without degradation in highly acidic or basic mobile phases, such as 1 M NaOH or H₂SO₄.
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
Oligonucleotide Purification
The properties of the PRP-C18 make it a superior column in the field of oligonucleotide purifications, providing:
Applications:
Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides
Examples of analytes that can be separated on PRP-C18 columns:
| Hamilton Company | |
|---|---|
| Product Category | HPLC Columns |
| Product Number | 79680 |
| Product Name | PRP-C18, 5 µm, 100 Å, 2.1 x 150 mm PEEK |
| Column Type | Analytical |
| Stationary Phase/Separation Process | Reversed Phase |
| Construction Material | polymer |
| Packing Diameter | 5 µm (0.1969 mils) |
| Pore Diameter | 0.0100 µm (3.94E-4 mils) |
