The Hamilton PRP-C18 is a reversed-phase HPLC column designed for high-efficiency separations across a wide pH range (1-13) and temperatures above 100°C, with mechanical stability and chemical inertness that prevent stationary phase degradation. The 4.6 x 250 mm variant (part number 79677) features a 5 µm particle size and is suitable for demanding applications in pharmaceutical, clinical, and life sciences industries. The column supports fast gradient elution, high pH operation, and regeneration with 1 M NaOH or H2SO4 without loss of efficiency. Thermal performance improves at elevated temperatures, with plate counts more than doubling at 80°C compared to ambient conditions.
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
Oligonucleotide Purification
The properties of the PRP-C18 make it a superior column in the field of oligonucleotide purifications, providing:
Applications:
Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides
Examples of analytes that can be separated on PRP-C18 columns:
| Hamilton Company | |
|---|---|
| Product Category | HPLC Columns |
| Product Number | 79677 |
| Product Name | PRP-C18, 5 µm, 100 Å, 4.6 x 250 mm |
| Column Type | Analytical |
| Stationary Phase/Separation Process | Reversed Phase |
| Construction Material | Stainless Steel |
| Packing Diameter | 5 µm (0.1969 mils) |
| Pore Diameter | 0.0100 µm (3.94E-4 mils) |
