Iron Staining Kit detects the presence of ferric iron in tissue. The iron is separated from protein by hydrochloric acid. The free ferric iron reacts with the potassium ferrocyanide to form an insoluble bright blue ferric ferrocyanide, or Prussian blue. A Nuclear Fast Red counterstain is applied to provide a pink to red contrasting background. Iron pigment stains bright blue. Heavy deposits of iron appear dark blue. Cytoplasm stains pink and nuclei stain pink to red.
| Ventana Medical Systems, Inc. | |
|---|---|
| Product Category | Organic Chemicals |
| Product Number | 860-009 |
| Product Name | Iron Staining Kit |