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Hamilton Company PRP-1 10 µm 4.1 x 250 mm 79427

Description
Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenze ne, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample. Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns. Applications: Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides Examples of analytes that can be separated on PRP-1 columns: Polycyclic aromatic hydrocarbons (PAH) Ionizable organic compounds Steroids Peptide fragments
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PRP-1 10 µm 4.1 x 250 mm - 79427 - Hamilton Company
Reno, NV, United States
PRP-1 10 µm 4.1 x 250 mm
79427
PRP-1 10 µm 4.1 x 250 mm 79427
Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenze ne, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample. Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns. Applications: Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides Examples of analytes that can be separated on PRP-1 columns: Polycyclic aromatic hydrocarbons (PAH) Ionizable organic compounds Steroids Peptide fragments

Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample.

Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.

Applications:

Organic compounds: small molecule (< 2,000 mw), pharmaceuticals, steroids, nucleic acids, vitamins, herbicides

Examples of analytes that can be separated on PRP-1 columns:

  • Polycyclic aromatic hydrocarbons (PAH)
  • Ionizable organic compounds
  • Steroids
  • Peptide fragments
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Technical Specifications

  Hamilton Company
Product Category HPLC Columns
Product Number 79427
Product Name PRP-1 10 µm 4.1 x 250 mm
Column Type Analytical
Stationary Phase/Separation Process Reversed Phase
Construction Material Stainless Steel
Packing Diameter 10 µm (0.3937 mils)
Pore Diameter 0.0100 µm (3.94E-4 mils)
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