Pkg of 20, IMAC affinity spin columns for metal affinity purification of proteins, for use with ProteinChip SELDI system
ProteinChip fractionation and purification products are used for applications before and after sample analysis on ProteinChip arrays. Before analysis, they can be used to fractionate a sample to reduce its complexity and increase the likelihood of identifying low-abundance proteins. After analysis and biomarker identification, these products, which have chromatographic properties that mimic the binding and elution characteristics of ProteinChip arrays, can be used to purify proteins discovered on array surfaces.
ProteinChip Spin Columns ProteinChip spin columns are used for small-scale purification of proteins using a microcentrifuge.
ProteinChip Q spin columns—anion exchange spin columns that fractionate samples based on pI through a quaternary amine functionality
ProteinChip CM spin columns—cation exchange spin columns that fractionate samples based on pI through a carboxymethyl functionality
ProteinChip IMAC spin columns—used for purifying recombinant polyhistidine-tagged proteins (when loaded with Ni2+) or phosphorylated proteins and peptides (when loaded with Ga3+ or Fe3+); IMAC sorbent chelates metal ions based on iminodiacetic acid functionality
Pkg of 20, IMAC affinity spin columns for metal affinity purification of proteins, for use with ProteinChip SELDI system
ProteinChip fractionation and purification products are used for applications before and after sample analysis on ProteinChip arrays. Before analysis, they can be used to fractionate a sample to reduce its complexity and increase the likelihood of identifying low-abundance proteins. After analysis and biomarker identification, these products, which have chromatographic properties that mimic the binding and elution characteristics of ProteinChip arrays, can be used to purify proteins discovered on array surfaces.
ProteinChip Spin Columns
ProteinChip spin columns are used for small-scale purification of proteins using a microcentrifuge.
- ProteinChip Q spin columns—anion exchange spin columns that fractionate samples based on pI through a quaternary amine functionality
- ProteinChip CM spin columns—cation exchange spin columns that fractionate samples based on pI through a carboxymethyl functionality
- ProteinChip IMAC spin columns—used for purifying recombinant polyhistidine-tagged proteins (when loaded with Ni2+) or phosphorylated proteins and peptides (when loaded with Ga3+ or Fe3+); IMAC sorbent chelates metal ions based on iminodiacetic acid functionality
