Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm
Bio-Rad offers ELISA substrate kits suitable for use in all microtitration enzyme immunoassays. The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution. Benefits of the AP system include an extremely linear reaction rate and low background values for accurate quantitation by ELISA.
Two convenient substrate systems are available for detection of HRP-labeled antibodies. Both the ABTS (2,2'-azino-di(3-eth
ylbenzthiazoline)-6-
sulfonic acid) peroxidase substrate kit and the TMB (3,3',5,5'-tetrameth
ylbenzidine) peroxidase substrate kit contain liquid reagents that are mixed just prior to use. No weighing or dissolving of hazardous chemicals is necessary. The single-component TMB peroxidase EIA substrate kit makes microplate assays convenient and fast.
Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm
Bio-Rad offers ELISA substrate kits suitable for use in all microtitration enzyme immunoassays. The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution. Benefits of the AP system include an extremely linear reaction rate and low background values for accurate quantitation by ELISA.
Two convenient substrate systems are available for detection of HRP-labeled antibodies. Both the ABTS (2,2'-azino-di(3-ethylbenzthiazoline)-6-sulfonic acid) peroxidase substrate kit and the TMB (3,3',5,5'-tetramethylbenzidine) peroxidase substrate kit contain liquid reagents that are mixed just prior to use. No weighing or dissolving of hazardous chemicals is necessary. The single-component TMB peroxidase EIA substrate kit makes microplate assays convenient and fast.
