Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, calcium form, 25 î¼m particle size, 4% cross linkage, pH range 5-9
Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:
In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size
For monosaccharide separations, ligand exchange is the primary mechanism and involves the binding of hydroxyl groups of the sugars with the fixed counterion of the resin. Ligand exchange is affected by the nature of the counterion (Pb2+, Ca2+, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups
Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 µm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.
Aminex columns are an industry standard for the analysis of carbohydrates. Bio-Rad offers a complete line of carbohydrate analysis columns optimized to provide high selectivity for a specific carbohydrate or carbohydrate class. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3–5 minutes.
Aminex HPX-87C Column This calcium-form column is the column of choice for most general sweetener analyses. It is optimized for analyzing monosaccharides and also provides class separation of di-, tri-, and tetrasaccharides. The 300 x 7.8 mm column is used primarily for the quantitation of glucose and fructose in high fructose corn syrup and for general monosaccharide analysis. The 250 x 4.0 mm column is appropriate for sugar alcohol separations.
Aminex HPX-87P Column This lead-form, 300 x 7.8 mm column is tailored for the separation of cellulose-derived monosaccharides. It is used in analyses of pentoses and hexoses in wood products, especially cellobiose, glucose, xylose, galactose, arabinose, and mannose. It also provides excellent resolution of sucrose, lactose, and fructose in dairy products.
Fast Carbohydrate Analysis Column This lead-form, 100 x 7.8 mm column is tailored for extremely fast separations of specific carbohydrates in samples where only certain components are of interest. It is optimized for 5-minute analyses of sucrose, glucose, galactose, and fructose.
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, calcium form, 25 μm particle size, 4% cross linkage, pH range 5-9
Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms:
- In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size
- For monosaccharide separations, ligand exchange is the primary mechanism and involves the binding of hydroxyl groups of the sugars with the fixed counterion of the resin. Ligand exchange is affected by the nature of the counterion (Pb2+, Ca2+, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups
Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 µm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.
Aminex columns are an industry standard for the analysis of carbohydrates. Bio-Rad offers a complete line of carbohydrate analysis columns optimized to provide high selectivity for a specific carbohydrate or carbohydrate class. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3–5 minutes.
Aminex HPX-87C Column
This calcium-form column is the column of choice for most general sweetener analyses. It is optimized for analyzing monosaccharides and also provides class separation of di-, tri-, and tetrasaccharides. The 300 x 7.8 mm column is used primarily for the quantitation of glucose and fructose in high fructose corn syrup and for general monosaccharide analysis. The 250 x 4.0 mm column is appropriate for sugar alcohol separations.
Aminex HPX-87P Column
This lead-form, 300 x 7.8 mm column is tailored for the separation of cellulose-derived monosaccharides. It is used in analyses of pentoses and hexoses in wood products, especially cellobiose, glucose, xylose, galactose, arabinose, and mannose. It also provides excellent resolution of sucrose, lactose, and fructose in dairy products.
Fast Carbohydrate Analysis Column
This lead-form, 100 x 7.8 mm column is tailored for extremely fast separations of specific carbohydrates in samples where only certain components are of interest. It is optimized for 5-minute analyses of sucrose, glucose, galactose, and fructose.
