Bio-Rad Laboratories, Inc. Affi-Gel Protein A Support 153-6153

Description
5 ml, ready-to-use protein A crosslinked agarose support  Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. Advantages include: High purity of IgGs High affinity for mammalian IgG High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH. With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.
  • Affi-Gel Protein A Support - 153-6153 - Bio-Rad Laboratories, Inc.
    Bio-Rad Laboratories, Inc.
Description
5 ml, ready-to-use protein A crosslinked agarose support  Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. Advantages include: High purity of IgGs High affinity for mammalian IgG High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH. With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.

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Affi-Gel Protein A Support - 153-6153 - Bio-Rad Laboratories, Inc.
Bio-Rad Laboratories, Inc.
Hercules, CA, USA
Affi-Gel Protein A Support
153-6153
Affi-Gel Protein A Support 153-6153
5 ml, ready-to-use protein A crosslinked agarose support  Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. Advantages include: High purity of IgGs High affinity for mammalian IgG High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH. With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.

5 ml, ready-to-use protein A crosslinked agarose support


Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.

Advantages include:

  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer

In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.

With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.

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Technical Specifications

  Bio-Rad Laboratories, Inc.
Product Category Gel Electrophoresis Equipment
Product Number 153-6153
Product Name Affi-Gel Protein A Support
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