Alcian Blue Staining Kit demonstrates weakly acidic mucopolysaccharides. At pH 2.5, Alcian Blue stains sulfated mucins, usually epithelial in origin (and usually PAS-negative), with some occurrences in connective tissue, e.g. in subcutaneous tissue in thyroid deficiency, (myxedema) and in myxomas. It also stains carboxylated sialomucins, found in the mucins of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated acid mucopolysaccharides. Some examples of components that stain positive with alcian blue are goblet cells, hyaluronic acid (found in umbilical cord and connective tisue in dermis), salivary glands, and gastric lining cells. Alcian Blue (pH 2.5) may be helpful in detecting genetic disorders of acid mucosubstance metabolism and collagen diseases where various acid mucosubstances are increased. The presence of acid mucosubstances tends to decline with advancing age.
Alcian Blue Staining Kit demonstrates weakly acidic mucopolysaccharides. At pH 2.5, Alcian Blue stains sulfated mucins, usually epithelial in origin (and usually PAS-negative), with some occurrences in connective tissue, e.g. in subcutaneous tissue in thyroid deficiency, (myxedema) and in myxomas. It also stains carboxylated sialomucins, found in the mucins of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated of submaxillary glands, small intestine and upper colon, and sulfated and carboxylated acid mucopolysaccharides. Some examples of components that stain positive with alcian blue are goblet cells, hyaluronic acid (found in umbilical cord and connective tisue in dermis), salivary glands, and gastric lining cells. Alcian Blue (pH 2.5) may be helpful in detecting genetic disorders of acid mucosubstance metabolism and collagen diseases where various acid mucosubstances are increased. The presence of acid mucosubstances tends to decline with advancing age.
