1.5 ml, 5x iProof PCR buffer for GC-rich templates
iProof high-fidelity DNA polymerase comprises a unique Pyrococcus-like proofreading enzyme fused to a dsDNA binding protein, Sso7d. This results in a thermostable polymerase that accurately amplifies long products from a variety of DNA templates. iProof polymerase is available in three convenient formats: a stand-alone enzyme, an easy-to-use master mix, and a PCR kit complete with controls. In comparison to reactions using Taq polymerase, all three formats feature:
High fidelity — iProof DNA polymerase is 52-fold more accurate
Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
1.5 ml, 5x iProof PCR buffer for GC-rich templates
iProof high-fidelity DNA polymerase comprises a unique Pyrococcus-like proofreading enzyme fused to a dsDNA binding protein, Sso7d. This results in a thermostable polymerase that accurately amplifies long products from a variety of DNA templates. iProof polymerase is available in three convenient formats: a stand-alone enzyme, an easy-to-use master mix, and a PCR kit complete with controls. In comparison to reactions using Taq polymerase, all three formats feature:
- High fidelity — iProof DNA polymerase is 52-fold more accurate
- Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
- Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
