Bio-Rad Laboratories, Inc. SsoFast EvaGreen Supermix With Low ROX 172-5213

20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 2,000 x 20 µl reactions SsoFast™ EvaGreen® supermix with low ROX is a ROX-blended version of the high-performing SsoFast EvaGreen supermix that employs the patented Sso7d fusion protein technology for use in real-time PCR applications. This novel, engineered polymerase, combined with optimized buffer and EvaGreen dye, produces robust qPCR results in less time with increased speed, reliability, and sensitivity under fast thermal cycling conditions. Key Features and Benefits Fast qPCR results — Sso7d fusion polymerase's stringent hot-start and rapid polymerization kinetics provide consistent qPCR results under fast cycling conditions (approximately 40 min) Preblended with ROX — streamlined use with real-time PCR systems that require ROX for fast runs Minimal PCR inhibition — highly tolerant polymerase and EvaGreen dye exhibit low PCR inhibition and provide better performance with fast-cycling protocols Single copy detection — higher concentrations generate larger fluorescent signals to provide increased sensitivity Broad dynamic range — data illustrate linear results over six orders of magnitude on real-time PCR systems Applications and Uses Dye-based qPCR detection PCR and real-time PCR analysis Nucleic acid amplification and expression profiling ROX-dependent real-time PCR detection Gene expression analysis Genotyping studies Genomics-related applications