Alpha Diagnostic International, Inc. Mouse Preadipocyte factor-1 (Pref-1) control/blocking peptide # 2 PREF12-P

Mouse Preadipocyte factor-1 (Pref-1) control/blocking peptide # 2 General Information Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein is implicated in inhibiting preadipocytes differentiation. Pref-1 (mouse 385-aa, rat 383-aa, human 383-aa, chromosome 14q32), also known as fetal anti SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Mouse Form Antigen/blocking Peptide Antigen 16-aa peptide of mouse Pref1/Dlk (protein accession #Q09163, refs 1) ; Designated (PREF12-P or control peptide) SpCrossreactivity Mouse PREF12-P sequence is 93% conserved in human and rat, and 87% in bovine Pref-1. Since the epitope of PREF12-S antibody is located near the cytoplasmic C-terminus, this antibody will not detect the ectodomain/ soluble forms of Pref-1. Antibody cross-reactivity in various other species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.