Alpha Diagnostic International, Inc. Rat/Human Orexin-A Control/blocking peptide # 1 OXA11-P

Rat/Human Orexin-A Control/blocking peptide # 1 General Information Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1-2). Orexin-A and Orexin-B are derived from the same pr SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Rat/Human Form Antigen/blocking Peptide Antigen 18-aa peptide of mouse Orexin-A (gene accession # 055232) (designated as OXA11-P, control peptide) conjugated to KLH. The peptide has two disulfides (Cys 6-Cys 12 & Cys 7-Cys 14). ; epitope location ~ N-terminus SpCrossreactivity Mouse OXA11-P control peptide is 100% identical in rat, pig, ovine, bovine, dog, human and 81% in chicken. Anti-OXA11 reacts with the full-length (1-33 aa) Orexin-A. The OXA11-P epitope is not found in Orexin-B. Since Orexin-A and Orexin?B are derived from an unprocessed pre-proorexin (130 aa), the anti-Orexin-A antibodies may also react with pre-pro orexin. Antibodies made to prepro-orexin A are also available. Antibody crossreactivity in various species is not established For research use only, not for diagnostic or therapeutic use.