Alpha Diagnostic International, Inc. Nitrated Mol Wt Std for Western blot NITT12-C

Nitrated Mol Wt Std for Western blot General Information Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2.-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration o SpeciesReactivity All species Form Protein Control Antigen #NITT12-C (10-20 blots) has a mixture of mol. Wt calibrated proteins (superoxide dismutase, ~16 kDa, occasionally a dimmer of ~32 kDa may also be seen; BSA ~66 kDa; and muscle myosin ~215 kDa) that were nitrated with peroxynitrite for use in Western blotting using the polyclonal anti-nitrotyrosine. Not recommended for use with monoclonal anti-nitrotyrosine. NITT12-C is supplied in SDS-PAGE sample buffer (reduced) for Western. Load 10 ul/lane of NITT12-C for good visibility with antibody Cat # NITT12-A or # NITT13-S For research use only, not for diagnostic or therapeutic use.