Human BACE/Asp2 substrate (wild type) APP590-603
General Information Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as -secretase. BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins. At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2,
SpeciesReactivity Human
Form Pure Peptide
Antigen -Secretase Substrate (Swedish) Human APP590-603 Acetyl-EISEVNL DA EFR HD-amide
SpCrossreactivity Purified BACE/Asp2 cleaves APP and synthetic APP peptide substrates at the -secretase site, and the rate of cleavage is increase 10-fold by a Swedish type mutation associated with early onset of Alzheimer?s disease. BACE has an acidic optimum (pH 4.0). Typically, purified BACE/Asp2 prepared by membrane solubilization in 25 mM Tris, pH 8.0, containing 50 mM -octylglucoside retained the enzyme activity. Enzymatic reactions are performed in 50 mM 2-(N-morpholino)etha
ne-sulfomate, pH 5.5, containing 70 uM peptide substrate and the reaction performed at 37oC [see details in Yan et al (1999) and Lin et al (1999)]. Reaction products are then analyzed by reverse phase HPLC.
For research use only, not for diagnostic or therapeutic use.
Human BACE/Asp2 substrate (wild type) APP590-603
General Information
Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as -secretase. BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins. At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2,
SpeciesReactivity
Human
Form
Pure Peptide
Antigen
-Secretase Substrate (Swedish) Human APP590-603 Acetyl-EISEVNL DA EFR HD-amide
SpCrossreactivity
Purified BACE/Asp2 cleaves APP and synthetic APP peptide substrates at the -secretase site, and the rate of cleavage is increase 10-fold by a Swedish type mutation associated with early onset of Alzheimer?s disease. BACE has an acidic optimum (pH 4.0). Typically, purified BACE/Asp2 prepared by membrane solubilization in 25 mM Tris, pH 8.0, containing 50 mM -octylglucoside retained the enzyme activity. Enzymatic reactions are performed in 50 mM 2-(N-morpholino)ethane-sulfomate, pH 5.5, containing 70 uM peptide substrate and the reaction performed at 37oC [see details in Yan et al (1999) and Lin et al (1999)]. Reaction products are then analyzed by reverse phase HPLC.
For research use only, not for diagnostic or therapeutic use.
