Alpha Diagnostic International, Inc. Recombinant purified Human Angiotensin converting enzyme protein 2 (ACE2) WB +ve control ACE22-C

Recombinant purified Human Angiotensin converting enzyme protein 2 (ACE2) WB +ve control General Information ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% seq homology to N- and C-terminal domains of ACE. However, in contrast t SpeciesReactivity Human Form Protein Control Antigen Human ACE-2 (1-740aa, glycosylated secreted protein of MW ~120kDa, predicted MW 85 kDa) with His tag was expressed in mouse myeloma cell line NS0 and purified >90% (SDS-PGE). Human ACE-2 protein for WB +ve control, Cat # ACE22-C, is formulated in SDS-PAGE sample buffer (reduced). This preparation is biologically inactive. It is not suitable for ELISA or other applications where native protein is required. It is supplied in 100 ul/vial. For WB, heat once and load 10 ul/lane and visualize with appropriate antibodies. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Angiogenin protein is ~14 kda. Store frozen in suitable aliquots. Do not freeze, thaw, or hear repeatedly. SpCrossreactivity Human ACE22-P control peptide is 95% conserved in mouse ACE-2, respectively. Antibody cross-reactivity in various species is not known. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.