Alpha Diagnostic International, Inc. Goat IgG (total) ELISA Kit, 96 tests, Quantitative 7520

Goat IgG (total) ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Goat Form Stds=0-400 ug/ml ; Sample=20 ul; Sensitivity= 250 pg/ml; 75 min assay; Quantitative, Competitive assay Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Goat IgG coated strip plate (8 wells x 12 strips) 7521 2. Goat IgG Std A (0.5 ug/ml), 0.250 ml 7522 3. Goat IgG Std B (3 ug/ml), 0. 250 ml 7523 4. Goat IgG Std C (10 ug/ml), 0. 250 ml 7524 5. Goat IgG Std D (50 ug/ml), 0. 250 ml 7525 6. Goat IgG Std E (100 ug/ml), 0. 250 ml 7526 7. Goat IgG Std F (400 ug/ml), 0. 250 ml 7527 8. Sample Diluent (20X), 10 ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. 100x Anti-Goat IgG-HRP Conjugate ,0.12 ml 7528 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-7520 SpCrossreactivity The antibodies used in the kit are not specific any specific IgG (IgG1, 2 etc) and may react with most IgGs. However, no significant reactivity is observed with IgA, IgM, or other serum proteins. Goat IgG ELISA kit reactivity was tested with the following animal serum IgG at concentrations of 100ug/ml: Sheep IgG (7%), Human IgG (2%), and Bovine IgG (2%), showed crossreactivity. Rat, Mouse, Monkey, Pig, Rabbit, Guinea Pig, Hamster, and Chicken serum IgG showed less than 1% crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.