Alpha Diagnostic International, Inc. Mouse Anti-Histones Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative 5610

Mouse Anti-Histones Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are SpeciesReactivity Mouse Form Stds= 0-4000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 125 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Histone Antigen Coated strip plate ( 8 wells x 12 strips) 5611 2. Mouse Ig?s std A (0 ng/ml), 0.650 ml 5612 3. Mouse Ig?s std B (250 ng/ml), 0.650 ml 5613 4. Mouse Ig?s std C (500 ng/ml), 0.650 ml 5614 5. Mouse Ig?s std D (1000 ng/ml), 0.650 ml 5615 6. Mouse Ig?s std E (2000 ng/ml), 0.650 ml 5616 7. Mouse Ig?s std F (4000 ng/ml), 0.650 ml 5617 8. Goat Anti-mouse IgG HRP conjugate 100X (H+L), 0.12 ml. 5618 9. Sample Diluent (10x), 10 ml SD-10 10. Wash Solution (100X) 10 ml WB-100 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual. M-5610 SpCrossreactivity Purified histones used in this kit as antigen contains all bands associated with subclasses f1, f2a1, f2a2, f2b, and f3. Anti-histone antibodies, as determined by this kit, will not distinguish between these histone subisotypes. This kit will measure anti-histones toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.