Ventana Medical Systems, Inc. Jones H&E Staining Kit 860-019

Jones Staining Kit demonstrates capillary basement membrane and is a modification of Jones Methanamine Silver procedure. Methanamine in this stain is used to demonstrate the carbohydrate component of basement membrane. Periodic acid is used to oxidize carbohydrates to aldehyde groups. Jones Silver A, a silver nitrate solution, provides the silver ions. Jones Silver B Stains for Connective Tissue: provides the alkaline conditions that reduce the silver ions to metallic silver. Toner reagent contains gold chloride to form a Elastic, Jones, Jones Light Green, more stable gold complex and remove the yellow tones from the tissue. Fixer, with thiosulfate, stops the reaction and removes Trichrome II Blue, Trichrome III Blue, any unreduced silver from the section. The purpose of this stain is primarily to distinguish pathological abnormalities in kidney Trichrome III Green, Reticulum II diseases. Hematoxylin and Eosin Counterstain are applied to provide contrasting background. The Jones Staining Kit stains basement membrane black against a light pink background. Basement membranes appear as black ink drawn lines and these fine lines should be seen in the glomeruli of the kidney.