Diastase is an enzyme which selectively digests (breaks down) glycogen. It is used to remove glycogen from tissue. This produces "negative PAS" staining of the glycogen. Any remaining PAS staining after diastase digestion must be due to non-glycogen protein-bound neutral polysaccharides, such as mucin or fungus. Diastase for PAS is an accessory reagent that is used with the PAS Staining Kit to a) identify glycogen with certainty, by its removal and/or b) eliminate it from the tissue section when it is interfering with the identification of other PAS-positive tissue structures. Because the glycogen is removed, the magenta staining is absent, but the nuclei still stain light purple with the Hematoxylin Counterstain.
Note: This is not a stand-alone reagent; it must be used in conjunction with PAS Staining Kit.
| Ventana Medical Systems, Inc. | |
|---|---|
| Product Category | Organic Chemicals |
| Product Number | 860-004 |
| Product Name | Diastase Kit |
