Hettich Instruments, LP Hettich Hematocrit 210
Benchtop Centrifuge

Hematocrit determination with standard capillaries is carried out with 24-place rotor no. 2076. Each capillary is secured within its own chamber and supported by a holding tray. This holding tray serves to contain glass shards and any leakage should a tube break. It is easy to clean and disposable in the case of glass breakage. The lid of rotor 2076 is cover and evaluation disc in one. Capillaries, to be used for quantitative buffy coat analysis are centrifuged in 20-place rotor no. 2056 prior to further analysis. Today, determining hematocrit values using centrifugation is still acknowledged as the Gold Standard (cf. DIN 58933-1*). In fact, the main area of application for hematocrit centrifuges is the determination of volume fraction of erythrocytes in blood. For this purpose, blood in hematocrit capillaries is centrifuged until maximum packed cell volume has been reached. Following centrifugation, the hematocrit value can be read using a special evaluation disc. Preparing blood for photometric determination of bilirubin content in plasma and centrifuging special capillaries for quantitative Buffy Coat Analysis are further areas of application for this special-purpose centrifuge. DIN 58933-1: Hematology - Procedure for determining the volume fraction of erythrocytes (packed cell volume) in blood - Part 1: Reference method based on centrifugation At 16,060 g sedimentation is completed in just a little over 6 minutes. At any rate, a run time of 10 minutes is sufficient. The rotor comes to a braked standstill.