The Hamilton PRP-C18 HPLC column, part number 79675, is a 4.6 x 50 mm reversed-phase column with 5 µm particle size, designed for high-efficiency separations across a wide pH range (1-13) and temperatures up to 100°C. It features a rigid, inert stationary phase that resists stripping, bleeding, or dissolution under extreme mobile phase conditions, enabling reliable performance and extended column life. The column supports fast gradient elution and high pH separations, improving resolution for basic compounds and reducing run times without degradation. It is compatible with aggressive regeneration using 1 M NaOH or H₂SO₄.
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (> 100°C), does not shrink or swell, and is completely inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
Oligonucleotide Purification
The properties of the PRP-C18 make it a superior column in the field of oligonucleotide purifications, providing:
Applications:
Organic compounds: small molecules (< 2,000 mw), pharmaceuticals, steroids, halides, vitamins, amino acid analysis, herbicides
Examples of analytes that can be separated on PRP-C18 columns:
| Hamilton Company | |
|---|---|
| Product Category | HPLC Columns |
| Product Number | 79675 |
| Product Name | PRP-C18, 5 µm, 100 Å, 4.6 x 50 mm |
| Column Type | Analytical |
| Stationary Phase/Separation Process | Reversed Phase |
| Construction Material | Stainless Steel |
| Packing Diameter | 5 µm (0.1969 mils) |
| Pore Diameter | 0.0100 µm (3.94E-4 mils) |
