The PRP-h5 HPLC column is a polymeric reversed-phase column designed for oligonucleotide purification and protein separations. It features a 4.6 mm inner diameter and 150 mm length, with a 5 µm particle size and 300 Å pore size, constructed from cross-linked poly(styrene-co-divi
The PRP-h5 utilizes the PRP-3 as its base with a pentafluorinated modification, making it more hydrophobic in nature. The PRP-h5, with its functionality derived from a pentafluorinated polymer bead, delivers a selectivity difference from standard silica C18 stationary phases. This gives chromatographers the desired retention characteristics necessary for certain sample types. This difference is especially pronounced for halogenated solutes.
Unlike silica-based C8 or C18 columns, PRP-h5 has no stationary phase coating. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many silica-based C8 or C18 columns.
Applications
Organic compounds: macromolecules (> 2,000 mw), pharmaceuticals, protein digests, tryptic digests, proteomics
| Hamilton Company | |
|---|---|
| Product Category | HPLC Columns |
| Product Number | 79272 |
| Product Name | PRP-h5 5 µm 4.6 x 150 mm |
| Column Type | Analytical |
| Stationary Phase/Separation Process | Reversed Phase |
| Construction Material | Stainless Steel |
| Packing Diameter | 5 µm (0.1969 mils) |
| Pore Diameter | 0.0300 µm (0.0012 mils) |
