200 x 50 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 10 mM Mg2+, ROX reference dye
iTaq supermix with ROX is formulated for optimal results in real-time qPCR assays with any real-time detection chemistry. This formula yields high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification over at least six orders of magnitude. The supermix is preblended with iTaq DNA polymerase, optimized buffer for probes-based detection, nucleotides, and ROX passive reference dye.
Key Features
Validated for use on all ROX-dependent real-time thermal cycler platforms and with all real-time detection chemistries
Able to perform duplex qPCR in a single reaction
Convenient 1-tube formulation, preblended with ROX to correct for inter-well signal variation
200 x 50 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 10 mM Mg2+, ROX reference dye
iTaq supermix with ROX is formulated for optimal results in real-time qPCR assays with any real-time detection chemistry. This formula yields high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification over at least six orders of magnitude. The supermix is preblended with iTaq DNA polymerase, optimized buffer for probes-based detection, nucleotides, and ROX passive reference dye.
Key Features
- Validated for use on all ROX-dependent real-time thermal cycler platforms and with all real-time detection chemistries
- Able to perform duplex qPCR in a single reaction
- Convenient 1-tube formulation, preblended with ROX to correct for inter-well signal variation
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