20 ml, 2,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers
iTaq™ Fast SYBR® Green supermix with ROX is a 2x ready-to-use reaction mix formulated to deliver maximum efficiency, sensitivity, and specificity and a robust fluorescent signal under fast or standard thermal cycling conditions. Designed specifically for use on the ABI 7500 fast or standard real-time PCR system and Stratagene real-time PCR systems, this supermix is preblended with iTaq DNA polymerase, optimized buffer, nucleotides, SYBR® Green I dye, and ROX passive reference dye for normalization of fluorescent signal and to correct for well-to-well optical variations.
Key Features and Benefits
Effective across a broad range of concentrations — data illustrate linear results over six orders of magnitude for detecting gene targets with high sensitivity
Versatile and fast performance — reproducible, highly uniform results across a wide range of template concentrations under both fast and standard cycling protocols. The rapid activation and polymerization kinetics permit fast qPCR results in less than 40 min
Simple protocol — convenient one-tube formulations enable you to obtain specific, sensitive, and fast qPCR results with ease
Applications and Uses
PCR and real-time PCR analysis
Nucleic acid amplification and expression profiling
ROX-dependent real-time PCR detection
Gene expression analysis
Genotyping studies
Genomics-related applications
20 ml, 2,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers
iTaq™ Fast SYBR® Green supermix with ROX is a 2x ready-to-use reaction mix formulated to deliver maximum efficiency, sensitivity, and specificity and a robust fluorescent signal under fast or standard thermal cycling conditions. Designed specifically for use on the ABI 7500 fast or standard real-time PCR system and Stratagene real-time PCR systems, this supermix is preblended with iTaq DNA polymerase, optimized buffer, nucleotides, SYBR® Green I dye, and ROX passive reference dye for normalization of fluorescent signal and to correct for well-to-well optical variations.
Key Features and Benefits
- Effective across a broad range of concentrations — data illustrate linear results over six orders of magnitude for detecting gene targets with high sensitivity
- Versatile and fast performance — reproducible, highly uniform results across a wide range of template concentrations under both fast and standard cycling protocols. The rapid activation and polymerization kinetics permit fast qPCR results in less than 40 min
- Simple protocol — convenient one-tube formulations enable you to obtain specific, sensitive, and fast qPCR results with ease
Applications and Uses
- PCR and real-time PCR analysis
- Nucleic acid amplification and expression profiling
- ROX-dependent real-time PCR detection
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
