500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.
Key Features and Benefits
Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
Use with different cell types — compatible with both suspension and adherent cell cultures
500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.
Key Features and Benefits
- Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5–10 min
- Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
- Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
- Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
- Use with different cell types — compatible with both suspension and adherent cell cultures
