25 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
The iScript™ Select cDNA synthesis kit is a sensitive reverse transcription system offering flexible priming strategies. Unlike our iScript cDNA synthesis kit and iScript reverse transcription supermix, this kit comes with multiple reaction components (5x buffer, oligo(dT), random primers, proprietary gene-specific primer enhancer solution, and water) that must be combined during reaction setup.
Key Features and Benefits
Have flexibility in mRNA priming strategy — oligo(dT), random primer, and gene specific primer enhancer solution provided in separate tubes
Synthesize long cDNAs up to 8 kb — quality controlled for reliable synthesis of cDNA >6 kb in length
Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
25 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
The iScript™ Select cDNA synthesis kit is a sensitive reverse transcription system offering flexible priming strategies. Unlike our iScript cDNA synthesis kit and iScript reverse transcription supermix, this kit comes with multiple reaction components (5x buffer, oligo(dT), random primers, proprietary gene-specific primer enhancer solution, and water) that must be combined during reaction setup.
Key Features and Benefits
- Have flexibility in mRNA priming strategy — oligo(dT), random primer, and gene specific primer enhancer solution provided in separate tubes
- Synthesize long cDNAs up to 8 kb — quality controlled for reliable synthesis of cDNA >6 kb in length
- Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
- Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription
