100 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2, SYBR® Green I, 20 nM fluorescein
iQ™ SYBR® Green supermix is formulated to achieve optimal results for a wide array of real-time PCR applications, with the convenience of SYBR Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR® Green I dye for sensitive and accurate detection, a hot-start iTaq™ DNA polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green® I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ™ SYBR® Green supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.
Key Features and Benefits
Increased throughput — requires minimal preparation since only template and primers are needed
High sensitivity and accuracy — the antibody-mediated, hot-start iTaq DNA polymerase minimizes the amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
High specificity over a broad dynamic range — the combination of the iQ™ SYBR® Green supermix with the iScript™ cDNA synthesis kit delivers effective results over a broad dynamic range of cDNA
Applications and Uses
PCR and real-time PCR analysis
Nucleic acid amplification and expression profiling
Gene expression analysis
Genotyping studies
Genomics-related applications
100 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2, SYBR® Green I, 20 nM fluorescein
iQ™ SYBR® Green supermix is formulated to achieve optimal results for a wide array of real-time PCR applications, with the convenience of SYBR Green I detection chemistry. This preblended 2x solution contains an optimized concentration of SYBR® Green I dye for sensitive and accurate detection, a hot-start iTaq™ DNA polymerase, a proprietary buffer and stabilizers specifically optimized for qPCR using SYBR Green® I, and qPCR-qualified dNTPs. This supermix also contains fluorescein, which is used as a passive reference dye for well-factor collection on certain real-time PCR detection systems. Use of iQ™ SYBR® Green supermix minimizes preparation time, produces reliable results for real-time PCR assays, and provides precise linear detection over six orders of magnitude.
Key Features and Benefits
- Increased throughput — requires minimal preparation since only template and primers are needed
- High sensitivity and accuracy — the antibody-mediated, hot-start iTaq DNA polymerase minimizes the amplification of nonspecific PCR products and allows sensitive and accurate detection of as few as 10 copies of template
- High specificity over a broad dynamic range — the combination of the iQ™ SYBR® Green supermix with the iScript™ cDNA synthesis kit delivers effective results over a broad dynamic range of cDNA
Applications and Uses
- PCR and real-time PCR analysis
- Nucleic acid amplification and expression profiling
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
