50 preps, protein preparation and fractionation kit for most membrane proteins, temperature dependent differential solubilization with Triton X-114
The ReadyPrep protein extraction kit (membrane I) uses temperature-dependen
t phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.
The ReadyPrep protein extraction kit (membrane I):
Isolates proteins associated with membranes or containing two transmembrane domains
Does not require the use of ultracentrifugation or density gradients
Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF
References Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607. Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.
50 preps, protein preparation and fractionation kit for most membrane proteins, temperature dependent differential solubilization with Triton X-114
The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.
The ReadyPrep protein extraction kit (membrane I):
- Isolates proteins associated with membranes or containing two transmembrane domains
- Does not require the use of ultracentrifugation or density gradients
- Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
- Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF
References
Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.
