Bio-Rad Laboratories, Inc. Nucleic Acid Sample Loading Buffer 161-0767

10 ml, 5x DNA loading buffer, premixed, for nondenaturing TBE gels, pH 8.0 Premixed Electrophoresis Running Buffers Save preparation time and standardize your electrophoresis runs with premixed electrophoresis running buffers. They can be used with handcast or precast gels. Premixed buffers are made with Bio-Rad electrophoresis-puri ty reagents and are quality controlled to ensure reproducible results. The 5 L cube packages are economical and are designed with an easy-pour spout for convenience. They are compact and stackable to save bench space. Premixed Electrophoresis Buffer Composition Buffer 1x Formulation Application(s) 10x TBE 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.3 Nucleic acid electrophoresis/sequ encing; polyacrylamide or agarose gels 10x TBE extended range* 130 mM Tris, 45 mM boric acid, 2.5 mM EDTA, pH 8.3 Nucleic acid electrophoresis 50x TAE 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.0 Polyacrylamide or agarose gels * Extends buffer capacity for longer DNA sequencing runs. Buffers are made with 18 megaohm water. Premixed Electrophoresis Sample Buffers Simplify and standardize your sample loading with premixed electrophoresis sample buffers. The concentrated formulas allow these buffers to be used with both liquid and lyophilized samples. All premixed sample buffers are tested to ensure quality and consistency. Premixed Sample Buffer Composition Buffer Formulation Application(s) 1x TBE-urea sample buffer 89 mM Tris-HCl, pH 8.0, 89 mM boric acid, 2 mM EDTA, 7 M urea, 12% Ficoll, 0.01% bromophenol blue, 0.02% xylene cyanole FF Denaturing, ssDNA, RNA 5x nucleic acid sample buffer 50 mM Tris-HCl, pH 8.0, 25% glycerol, 5 mM EDTA, 0.2% bromophenol blue, 0.2% xylene cyanole FF Nondenaturing, dsDNA