Alpha Diagnostic International, Inc. Human IgE (Plasma), purified 20007-3P

Human IgE (Plasma), purified General Information Human plasma was used for the purification of IgE. IgG was prepared by ammonium sulfate, and column chromatography (Sephadex, and DEAE-ion exchange columns) and affinity chromatography. The resulting preparation was judged to be >96% pure by IEP and SDS SugApplications This preparation of is suitable for coating the ELISA plates or as a non-immune control for ELISA, dot blot, Western or IHC. Recommended testing concn. is approx. 1-10 ug/ml. It can laso be used to prepare immunoadorbent to remove or to purify antibodie SugSecAntibodies Secondary antibodies should be directed against the primary host (example: if primary antibodies are made in rabbit then the secondary should be directed against the rabbit. Secondary antibodies can be made in goat, sheep, mouse, chicken etc). ADI has v SpeciesReactivity Human Form Normal Control Ig's Antigen Pure Protein SpCrossreactivity Purified IgE produced a single arc against anti-human serum and anti-human IgE. It does not react with anti-human IgA, IgD, IgG and IgM in IEP assay MSDS The product may contain sodium azide (0.05%), or merthiolate (0.02%) or Proclin-300 (0.1%). Please see the details in data sheets. Please note that although the product is defined as not hazardous, it is still advisable to follow prudent laboratory practice For research use only, not for diagnostic or therapeutic use.