Human IgG F(ab')2 fragment, purified
General Information
SugApplications This preparation of is suitable for coating the ELISA plates or as a non-immune control for ELISA, dot blot, Western or IHC. Recommended testing concn. is approx. 1-10 ug/ml. It can laso be used to prepare immunoadorbent to remove or to purify antibodie
SugSecAntibodies Secondary antibodies should be directed against the primary host (example: if primary antibodies are made in rabbit then the secondary should be directed against the rabbit. Secondary antibodies can be made in goat, sheep, mouse, chicken etc). ADI has v
SpeciesReactivity Human
Form Normal Control Ig's
Antigen A healthy non-immunized population of normal Human was used to collect serum. This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresi
s resulted in a single precipitin arc against anti-Human IgG, anti-Human IgG F(ab?)2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
MSDS The product may contain sodium azide (0.05%), or merthiolate (0.02%) or Proclin-300 (0.1%). Please see the details in data sheets. Please note that although the product is defined as not hazardous, it is still advisable to follow prudent laboratory practice
For research use only, not for diagnostic or therapeutic use.
Human IgG F(ab')2 fragment, purified
General Information
SugApplications
This preparation of is suitable for coating the ELISA plates or as a non-immune control for ELISA, dot blot, Western or IHC. Recommended testing concn. is approx. 1-10 ug/ml. It can laso be used to prepare immunoadorbent to remove or to purify antibodie
SugSecAntibodies
Secondary antibodies should be directed against the primary host (example: if primary antibodies are made in rabbit then the secondary should be directed against the rabbit. Secondary antibodies can be made in goat, sheep, mouse, chicken etc). ADI has v
SpeciesReactivity
Human
Form
Normal Control Ig's
Antigen
A healthy non-immunized population of normal Human was used to collect serum. This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG, anti-Human IgG F(ab?)2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
MSDS
The product may contain sodium azide (0.05%), or merthiolate (0.02%) or Proclin-300 (0.1%). Please see the details in data sheets. Please note that although the product is defined as not hazardous, it is still advisable to follow prudent laboratory practice
For research use only, not for diagnostic or therapeutic use.
