Alpha Diagnostic International, Inc. Human Adiponectin (Acrp30) ELISA Kit, 96 tests, Quantitative 100-140-ADH

Human Adiponectin (Acrp30) ELISA Kit, 96 tests, Quantitative General Information Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In o SpeciesReactivity Human Form Stds=0-12 ng/ml ; Sample=50 ul; Sensitivity= 0.6 ng/ml; 225 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Urine, Culture medium KitContents 1. Anti-Human Acrp30 coated strip plate (8 wells x 12 strips) 100-141 2. Human Acrp30 Std. A (0 ng/ml), 0.500 ml 100-142A 3. Lyophilized Human Acrp30 Std. E (6 ng), 2 vials 4. Reconstitute with 0.5 ml dH20 100-142B 5. Biotin-Anti-hAcrp30 Detection Ab, (100x), 0.12 ml 100-143 6. (20x) Sample Diluent, 10ml SD-20 7. Wash Buffer (100X), 10 ml WB-100 8. (100x) Streptavidin-HRP conjugate, 0.12ml S-HRP100 9. TMB Substrate, 12 ml 80091 10. Stop solution, 12 ml 80101 11. Instruction Manual 100-140-ADH SpCrossreactivity Human adiponectin cross reactivity was tested with the following serum proteins at 8 ng/ml: Mouse Acrp30, Human gAcrp30, and Mouse gAcrp30 all had less than 1% cross reactivity. Cross reactivity was tested with the following animal serum (adiponectin) at 1:100 dilution: Monkey serum had significant reactivity(>100%). Mouse, Rat, Guinea Pig, Bovine, Sheep, and Goat (sera containing full length Acrp30 or gACrp30) all had less than 1% crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.