Beta-Lactamase, E.C.3.5.2.6, penicillin amido-beta-lactam hydrolase, isolated from the organism, Bacillus cereus 569/H9 Beta Lactamase is used to destroy/remove Beta-Lactam antibiotics. Has broad sprectrum of activity against both penicillins (penicillinase or Beta I activity) and cephalosporins (cephalosporinase or Beta II activity). BETA-LACTAMASE will inactivate the following antibiotics: Beta-Lactam: Benzylpenicillin, ampicillin, amoxycillin, carbenicillin, methicillin, cloxacillin, flucloxacillin. Cephalosporins: cephaloridine, cephalothin, cephalexin, cephazolin, cephradine*,cefuroxi
me*, cefoxitin*, ceftizoxime*, cefpiramide* (*require longer incubations for total inactivation). Carbapenems: Bacillus cereus Beta-Lactamse II has been reported to hydrolyse some carbapenems. Sterilty: The vialed products are bacteriologically sterile. Sterilization is via gamma irradiation, with no detectable growth in Nutrient Broth at 37 °C for fourteen days. The product is vialed to contain: >500 Beta I units / vial and >50 Beta II units/ vial. A Beta I unit is defined as the amount of enzyme that will catalyse the hydrolysis of 1.0 micromole of benzylpenicillin per minute at 25°C and pH 7.0. Beta II unit is defined as the amount of enzyme that will catalyse the hydrolysis of 1.0 micromole of cephalosporin C per minute at 25°C and pH 7.0. One unit of activity is defined as the amount of enzyme that will catalyze the hydrolysis of 1.0 micromole of penicillin (beta I) or cephalosporin (beta II) per minute at 25'C under the assay method conditions. The material is dispensed into each vial by volume, not by weight. The amount dispensed varies by batch and is based on the concentration (units) of B-lactamase I and II. The material is irradiated and loses some activity due to the irradiation and this is taken into account when determining the final concentration of B-lactamase I and II per vial.
Beta-Lactamase, E.C.3.5.2.6, penicillin amido-beta-lactam hydrolase, isolated from the organism, Bacillus cereus 569/H9 Beta Lactamase is used to destroy/remove Beta-Lactam antibiotics.
Has broad sprectrum of activity against both penicillins (penicillinase or Beta I activity) and cephalosporins (cephalosporinase or Beta II activity).
BETA-LACTAMASE will inactivate the following antibiotics: Beta-Lactam: Benzylpenicillin, ampicillin, amoxycillin, carbenicillin, methicillin, cloxacillin, flucloxacillin. Cephalosporins: cephaloridine, cephalothin, cephalexin, cephazolin, cephradine*,cefuroxime*, cefoxitin*, ceftizoxime*, cefpiramide* (*require longer incubations for total inactivation). Carbapenems: Bacillus cereus Beta-Lactamse II has been reported to hydrolyse some carbapenems. Sterilty: The vialed products are bacteriologically sterile. Sterilization is via gamma irradiation, with no detectable growth in Nutrient Broth at 37 °C for fourteen days.
The product is vialed to contain: >500 Beta I units / vial and >50 Beta II units/ vial. A Beta I unit is defined as the amount of enzyme that will catalyse the hydrolysis of 1.0 micromole of benzylpenicillin per minute at 25°C and pH 7.0.
Beta II unit is defined as the amount of enzyme that will catalyse the hydrolysis of 1.0 micromole of cephalosporin C per minute at 25°C and pH 7.0.
One unit of activity is defined as the amount of enzyme that will catalyze the hydrolysis of 1.0 micromole of penicillin (beta I) or cephalosporin (beta II) per minute at 25'C under the assay method conditions.
The material is dispensed into each vial by volume, not by weight. The amount dispensed varies by batch and is based on the concentration (units) of B-lactamase I and II. The material is irradiated and loses some activity due to the irradiation and this is taken into account when determining the final concentration of B-lactamase I and II per vial.
