Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.
Use: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 μg/ml. Commonly used concentrations for selection are 200 μg/ml for mammalian cells, 20-200 μg/ml for plant cells & bacteria cells and 200-1000 μg/ml for fungi. Your optimum concentration should be tested experimentally.
Interested in our Hygromycin B Blog of Hygromycin's most Frequently Asked Questions (FAQS)?? CLICK HERE.
For a full listing of hygromycin B formulations.CLICK HERE.
********************
Seeking a unique, hard to source, or cutting edge ANTIBIOTIC? A.G.Scientific's comprehensive Antibiotic E-Booklet contains over 160 novel antibiotics including: Antimicrobial, antifungal, anticancer, immunosuppressive, antivial or antiparasitic agents.
Download your FREE Antibiotic E-booklet NOW. Click here.
Seeking bulk pricing on bulk or multiple vials quantities. All delivered over a set period of time?
Discounts are readily available on all three options. Submit your inquiry NOW. Click here.
********************
| A.G. Scientific, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | H-1012-CON |
| Product Name | Hygromycin B, Concentrated Solution |
