Ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
| A.G. Scientific, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | C-2234 |
| Product Name | Caspase-13 Substrate LEED-AFC |
