Benzamidine is a reversible competitive inhibitor of trypsin, trypsin-like enzymes and serine proteases. As a general inhibitor it can be used at 1 mM. It is sensitive to oxidation and should be prepared fresh in deionized water up to 50 mg/ml (heating may be necessary for solubilization).
It is often used as a ligand in protein crystallography to prevent proteases from degrading a protein of interest; the triangular diamine group at the bottom gives it a very obvious 'stick-man' shape which shows up in difference density maps.
Benzamidine (0.5 M)
Cold Spring Harb Protoc, Benzamidine (0.5 M). 2007; 2007: pdb.rec10928
Kinase buffer with benzamidine, 1X
Cold Spring Harb Protoc, Kinase buffer with benzamidine, 1X. 2007; 2007: pdb.rec10776.
| A.G. Scientific, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | B-1313 |
| Product Name | Benzamidine, Hydrochloride |