TCI America Melatonin M1105

Melatonin / N-Acetyl-5-methoxytryptamine
Living organisms acquire energy by bringing oxygen into their bodies. However, they can also be damaged by oxidative stress. Hence they maintain complex chemical systems of multiple types of antioxidants, which are chemical substances that protect biological materials against the effects of this stress. Human beings utilize antioxidants in a daily life as food additives to avoid their deterioration. Antioxidants in pharmacology are studied, particularly as treatments of stroke and nerve atrophy diseases. Antioxidants are also widely used as ingredients of functional foods. However, some clinical trials suggested that getting antioxidants during athletic exercise might be harmful. Functioning of the antioxidants remains to be investigated.
• Functions of Antioxidants
(1) Scavenging of active oxygen species and radicals
Scavenging of hydrogen peroxide (H2O2), free radicals such as the hydroxyl radical (•OH), the superoxide anion (O2^?), and the radicals they produce with biological molecules.
(2) Metal inactivation
Chelating metals to inactivate a metal such as iron which promotes oxidation
(3) Oxidase inhibition
(4) Protection of disulfide bond
Inhibition of the oxidative cleavage of disulfide bonds in the proteins.
• Classification of Antioxidants
Antioxidants are classified according to their solubility.
(1) Water-soluble antioxidants
Ascorbic acid (Vitamin C), Glutathione, Lipoic acid.
Typical properties: (a) Neutralization of reactive oxygen species, (b) Inhibition of the cleaving reaction of disulfide bonds.
(2) Fat-soluble antioxidants
α-Tocopherol (Vitamin E), gallates, resveratrol, polyphenols (e.g. ubiquinone, or coenzyme Q10), polyene compounds (e.g. β-Carotene).
Typical properties: Antioxidation of lipids through radical scavenging activity
• Storage Precautions
Solid antioxidants can be stored in a cool and dry place, unless otherwise specified. After opening, they should be stored under inert gases such as nitrogen or argon to prevent their oxidation. Since oily antioxidants can deteriorate faster than solids, they should be stored under an inert gas in a refrigerator. Refrigerated antioxidants should be warmed in a desiccator at room temperature under ambient atmosphere just before use without opening the cap. This operation can avoid moisture uptake of the reagents.

Melatonin / N-Acetyl-5-methoxytryptamine
In recent years, studies on nitric oxide (NO), one of the reactive oxygen species, have been actively conducted. It is well known that NO is produced in vivo from the guanidino nitrogen of arginine and oxygen by nitric oxide synthase (NOS), and plays an important role in various scenes such as vascular
relaxation, biological defense, neurotransmission modulation and immune system.
The following three types of compounds generate NO.1)
1) Compounds which act on an NO generating system and facilitate NO generation in vivo : e.g. Emodin, etc.
They activate the vascular endothelium and macrophages, etc., increasing NO generation.
2) Compounds which are converted into NO-releasing compounds via metabolism : e.g. nitric esters, nitrite esters, etc.
They react with thiols in vivo and release NO.
3) Compounds which directly release NO by itself : e.g. N-nitrosodiphenylamine, etc.
N-nitrosodiphenylamine releases NO by radical cleavage of the Nï¼NO bond when it is heated to reï¬ ux in benzene.
Popular analytical methods of NO include a chemiluminescent method, a spin trap method and the Griess method.2) The Griess method determines NO2ï¼, which is a metabolite of NO, while the chemiluminescent method and the spin trap method directly determine NO. Moreover, a simultaneous determination method of NO and NO2 using PTIO, the organic radical that selectively oxidizes NO, has been developed.3)