TCI America 1,2-Bis[2-[bis(carboxymethyl)amino]ethoxy]ethane E0805

Ethylene Glycol Bis(2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid [for Biochemical Research] / 1,2-Bis[2-[bis(carboxymethyl)amino]ethoxy]ethane
Photometric ReagentsWith the development of new analytical equipment and methods, for example MALDI-TOF-MS, an ever wider range of compounds has been identified by micro-analytical techniques and measured with high sensitivity. Modern analyses require high quality analytical reagents, since without them the results cannot be guaranteed. TCI supplies a variety of high quality analytical reagents for research use.

Ethylene Glycol Bis(2-aminoethyl Ether)-N,N,N',N'-tetraacetic Acid [for Biochemical Research] / 1,2-Bis[2-[bis(carboxymethyl)amino]ethoxy]ethane
In this section, proteases are categorized into three modes of actions: (1) serine protease; (2) cysteine protease; (3) metalloprotease.1)
Serine proteases are inhibited by AEBSF, benzamidine, etc.2) Cysteine proteases are inhibited by compounds which react with SH groups such as 2-iodoacetamide or 2-iodoacetic acid.3) Metalloproteases are inhibited by chelating agents, EDTA or 1,10-phenanthroline.4)
In the course of protein extraction, proteolysis is considered to be a major problem because it leads to decreasing yields. Addition of protease inhibitors helps to avoid the proteolysis and improves recovery of the desired protein.5) In the experiment of immunoprecipitation, protease inhibitors are also used to avoid decomposition of antigens or antibodies by proteolytic impurities.6) Protease inhibitors which are frequently used in biochemical research are illustrated in this section.
Thiol protease inhibitors, 2-iodoacetamide and 2-iodoacetic acid, cannot be used in protein extraction, because they form covalent bonds to the SH group of the proteins. In protein research, they are used as an alkylation reagent to derivatize the proteins which are extracted from an electrophoresis gel for mass spectrometric analysis.7)