TCI America 1-Benzyl-3-p-tolyltriazene [for Esterification] B0949

1-Benzyl-3-p-tolyltriazene [for Esterification] / 3-p-Tolyl-1-benzyltriazene
HPLC is used extensively as a means of detecting and determining trace components. In particular, it is effective in the case of non-volatile trace components in complex matrices such as biological substances. Labeling objective substances for analysis with labeling reagents appropriate for detection methods has been performed to obtain higher sensitivity and selectivity. A great number of labeling reagents have been reported for this purpose.
NBD- and DBD-derivatives developed by Imai, Toyo'oka and co-workers are excellent fluorescent labeling reagents with strong and long wave-length fluorescence resulting from their benzoxadiazole skeleton and they are used for analysis of various biological substances. NBD-Cl was the earliest fluorescence reagent which was applied to HPLC and its effectiveness for the secondary amines, such as proline, was reported. Imai and co-workers developed NBD-F1), in which the chlorine was replaced with fluorine, for HPLC analysis and obtained good results. Also, they developed DBD-F2) where a dimethylsulfamoyl group was introduced into the benzoxadiazole skeleton and used it for the analysis of amino acids by reversed phase HPLC. Because DBD-F itself is non-fluorescent, DBD-labeled amino acids can be detected and analyzed with high sensitivity. DBD-COCl3) reacts with a hydroxy group as well as amines and thiols, and forms a stable fluorescence adduct. In addition, NBD- and DBD-H4), in which a hydrazino group is introduced into the NBD-, DBD- skeleton, are used for analysis of carbonyl groups. NBD- and DBD-PZ5), in which a piperazino group is introduced, are used for analysis of carboxyl groups.

1-Benzyl-3-p-tolyltriazene [for Esterification] / 3-p-Tolyl-1-benzyltriazene
For GC analysis, the substance to be analyzed must be in the gaseous form. Because of this, the analysis of many organic compounds requires that injections, columns, and detectors are kept at relatively high temperatures. However, many organic compounds are thermally unstable. In such cases, it is necessary to convert the substance to be analyzed to a more volatile derivative appropriate for GC analysis. GC derivatizing reagents such as silylating, acylating, and alkylating reagents are used for this conversion.
Meanwhile, GC has been used for the analysis of biogenic substances. This generally requires the use of various derivatizing reagents. As an example, trimethylsulfonium hydroxide 1 can easily convert fatty acids, fatty acid esters, and glycerides into their methyl esters in a single step through the pyrolysis of the corresponding sulfonium salts 2.1) The low-boiling by-product, dimethyl sulfide, does not disturb the chromatographic separation of the samples. Therefore, the method using 1 is suitable for detection not only of longer chain fatty acids but also of relatively short chain fatty acids. 1 has been successfully applied for sample preparation in the analysis of fatty acids in microorganisms and human blood serum.2)