SPIE Dynamic and Agile Focusing in Microscopy: A Review ISBN: 9781510604438

This Spotlight introduces miniature varifocus elements and covers the optical equations needed to integrate them with microscopes. It reviews the current technological capabilities and limitations of different varifocus elements, such as deformable membrane mirrors, liquid crystal devices, liquid lenses, and vertical displacement microlenses. A discussion of varifocus elements demonstrated in widefield microscopes, confocal microscopes, two-photon microscopes, and optical coherence tomography is included. This book serves as a course module in electrical engineering and physics, while also benefiting biologists and chemists who desire deeper knowledge of this particular technology.