Applications:
Preparation of fluorescent or radioactively labeled RNA probes
Preparation of large RNA transcripts for analysis or in vitro translation
Amplification of RNA (in conjuction with AMV RT and Ribonuclease H.)
Preparation of anti-sense RNA
Preparation of ribozymes
Preparation of mRNA precursor for splicing
Preparation of ds RNA for RNA interference or silencing
Preparation of substrate in RNase protection assays
Preparation of template for genomic DNA sequencing
Preparation of RNA for studies of RNA secondary structure and RNA-protein interactions
Reagents supplied:
5X Reaction Buffer for T7 RNA Polymerase
1X Reaction Buffer Conditions
40 mM TrisHCl, pH 7.7
6 mM MgCl2
10 mM DTT
2 mM Spermidine
To be supplemented with
0.5 mM ATP, CTP, GTP and UTP
DNA template with T7 promoter
(20 nM of a linearized plasmid DNA or 40 ug/mL of a 3 Kb plasmid))
Incubate at 37ºC
| Life Sciences, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | T7G-70000 |
| Product Name | T7 RNA Polymerase in Glycerol |
