Life Sciences, Inc. AMV Reverse Transcriptase XL RTHSA35

Description
Applications For all AMV RT catalog numbers: cDNA Library production First strand cDNA synthesis for cloning RNA sequencing RT-PCR High-temperature cDNA Synthesis at up to 58ºC Reagent supplied, with a protocol for cDNA synthesis: AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45) At 1X concentration the magnesium ion concentration is only 5 mM. Under this condition, endogenous RNase H activity is minimal. 1X AMV RT Reaction buffer 25 mM TrisHCl, pH 8.3 @ 25ºC 50 mM KCl 2.0 mM DTT 5.0 mM MgCl2 Supplement with other components for cDNA synthesis Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using poly(rA)-d(T)12-18 as template primer. Unit Assay Conditions 0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT. AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics. Storage Conditions for AMV RT in Glycerol 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 50% glycerol, 0.2% Triton X-100 Storage Conditions for AMV RT in Trehalose 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 1.0 M Trehalose, 0.2% Triton X-100 Storage Temperature -20ºC for 3 months or -70ºC for 1.5 years
  • AMV Reverse Transcriptase XL - RTHSA35 - Life Sciences, Inc.
    Life Sciences, Inc.
Description
Applications For all AMV RT catalog numbers: cDNA Library production First strand cDNA synthesis for cloning RNA sequencing RT-PCR High-temperature cDNA Synthesis at up to 58ºC Reagent supplied, with a protocol for cDNA synthesis: AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45) At 1X concentration the magnesium ion concentration is only 5 mM. Under this condition, endogenous RNase H activity is minimal. 1X AMV RT Reaction buffer 25 mM TrisHCl, pH 8.3 @ 25ºC 50 mM KCl 2.0 mM DTT 5.0 mM MgCl2 Supplement with other components for cDNA synthesis Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using poly(rA)-d(T)12-18 as template primer. Unit Assay Conditions 0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT. AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics. Storage Conditions for AMV RT in Glycerol 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 50% glycerol, 0.2% Triton X-100 Storage Conditions for AMV RT in Trehalose 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 1.0 M Trehalose, 0.2% Triton X-100 Storage Temperature -20ºC for 3 months or -70ºC for 1.5 years

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AMV Reverse Transcriptase XL - RTHSA35 - Life Sciences, Inc.
Life Sciences, Inc.
St. Petersburg, FL, USA
AMV Reverse Transcriptase XL
RTHSA35
AMV Reverse Transcriptase XL RTHSA35
Applications For all AMV RT catalog numbers: cDNA Library production First strand cDNA synthesis for cloning RNA sequencing RT-PCR High-temperature cDNA Synthesis at up to 58ºC Reagent supplied, with a protocol for cDNA synthesis: AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45) At 1X concentration the magnesium ion concentration is only 5 mM. Under this condition, endogenous RNase H activity is minimal. 1X AMV RT Reaction buffer 25 mM TrisHCl, pH 8.3 @ 25ºC 50 mM KCl 2.0 mM DTT 5.0 mM MgCl2 Supplement with other components for cDNA synthesis Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using poly(rA)-d(T)12-18 as template primer. Unit Assay Conditions 0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT. AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics. Storage Conditions for AMV RT in Glycerol 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 50% glycerol, 0.2% Triton X-100 Storage Conditions for AMV RT in Trehalose 0.2 M potassium phosphate, pH 7.2 2.0 mM Dithiothreitol 1.0 M Trehalose, 0.2% Triton X-100 Storage Temperature -20ºC for 3 months or -70ºC for 1.5 years

Applications For all AMV RT catalog numbers: cDNA Library production First strand cDNA synthesis for cloning RNA sequencing RT-PCR High-temperature cDNA Synthesis at up to 58ºC Reagent supplied, with a protocol for cDNA synthesis: AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45) At 1X concentration the magnesium ion concentration is only 5 mM. Under this condition, endogenous RNase H activity is minimal. 1X AMV RT Reaction buffer 25 mM TrisHCl, pH 8.3 @ 25ºC 50 mM KCl 2.0 mM DTT 5.0 mM MgCl2 Supplement with other components for cDNA synthesis

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using poly(rA)-d(T)12-18 as template primer.

Unit Assay Conditions

0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT. AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics.

Storage Conditions for AMV RT in Glycerol

0.2 M potassium phosphate, pH 7.2
2.0 mM Dithiothreitol
50% glycerol,
0.2% Triton X-100

Storage Conditions for AMV RT in Trehalose

0.2 M potassium phosphate, pH 7.2
2.0 mM Dithiothreitol
1.0 M Trehalose,
0.2% Triton X-100

Storage Temperature

-20ºC for 3 months or -70ºC for 1.5 years

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Technical Specifications

  Life Sciences, Inc.
Product Category Biological Materials
Product Number RTHSA35
Product Name AMV Reverse Transcriptase XL
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