CSA Group Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography ISO 8128-2:1993

Description
The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothia zoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.
Description
The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothia zoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

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Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography - ISO 8128-2:1993 - CSA Group
Toronto, Ontario, Canada
Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography
ISO 8128-2:1993
Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography ISO 8128-2:1993
The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothia zoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 µg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

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Technical Specifications

  CSA Group
Product Category Standards and Technical Documents
Product Number ISO 8128-2:1993
Product Name Apple juice, apple juice concentrates and drinks containing apple juice - Determination of patulin content - Part 2: Method using thin-layer chromatography
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