Alpha Diagnostic International, Inc. Anti-Mouse Preadipocyte factor-1 (Pref-1) IgG # 2, aff pure PREF12-A

Anti-Mouse Preadipocyte factor-1 (Pref-1) IgG # 2, aff pure General Information Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein is implicated in inhibiting preadipocytes differentiation. Pref-1 (mouse 385-aa, rat 383-aa, human 383-aa, chromosome 14q32), also known as fetal anti SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Mouse SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen 16-aa peptide of mouse Pref1/Dlk (protein accession #Q09163, refs 1) ; Designated (PREF12-P or control peptide) SpCrossreactivity Mouse PREF12-P sequence is 93% conserved in human and rat, and 87% in bovine Pref-1. Since the epitope of PREF12-S antibody is located near the cytoplasmic C-terminus, this antibody will not detect the ectodomain/ soluble forms of Pref-1. Antibody cross-reactivity in various other species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.