Alpha Diagnostic International, Inc. Anti-Human NaPi-2/NPT2 antiserum # 6 NPT26-S

Anti-Human NaPi-2/NPT2 antiserum # 6 General Information Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine. Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney. At least 4 SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2477-seconda ry-antibodies-chicke n.htm SpeciesReactivity Human SugNegativeControl Non-immune Chicken IgG Cat# 20010-1 Form Antiserum Unpurified SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen Human Type-IIa, NPT2/Napi-3 is ~640 aa (chromosome 8) and mouse and rat 637 aa proteins. A 21 AA synthetic peptide (designated NPT26-P; control peptide) within the 1st extracellular loop (between TM1-2) of Human NPT2a (126-146 aa) was synthesized, conjugated to KLH SpCrossreactivity The NPT26-P peptide is 100% conserved in rat, mouse, ovine, opossum, 95% in rabbit, 85% in Zebra fish, and 71% in frog NPT2a. The NPT26-P sequence also has significant sequence homology (~65%) with mouse, rat, and human Type-IIb transporters. Therefore, antibodies may show some crossreactivity with Type2b NPT2. No significant sequence homology of NPT26-P is observed with other Pi-transporters (Type-I, III, or IV) transporters. Antibody crossreactivity in various species or isoform is not established For research use only, not for diagnostic or therapeutic use.