Alpha Diagnostic International, Inc. Anti-Human NaPi-2/NPT2 IgG #1, aff pure NPT21-A

Anti-Human NaPi-2/NPT2 IgG #1, aff pure General Information Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine. Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney. At least 4 SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Human SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen Human Type-IIa, NPT2/Napi-3 is ~640 aa (chromosome 8) and mouse and rat 637 aa proteins. A 20 AA synthetic peptide (designated NPT21-P; control peptide) within the 2nd extracellular loop (between TM3-4) of Human NPT2 (206-225 aa) was synthesized, conjugated to KLH SpCrossreactivity The NPT21-P peptide is 100% conserved in rat, mouse, sheep, 95% in opossum, and 90% in chicken, zebra fish, frog, and zebra fish NPT2a proteins. The NPT21-P sequence is also highly conserved (~90%) in mouse, human, and other species NPT2b/Type-Iib. Therefore, antibodies are expected to detect both Type2a and Type2b NPT2. No significant sequence homology of NPT21-P is observed with other Pi-transporters (Type-I, III, or IV) transporters. Antibody crossreactivity in various species is not established For research use only, not for diagnostic or therapeutic use.