Alpha Diagnostic International, Inc. Anti-Rat Rat Na-K-Cl Cotransporters 2 (NKCC2/BSC1) antiserum #1 NKCC21-S

Anti-Rat Rat Na-K-Cl Cotransporters 2 (NKCC2/BSC1) antiserum #1 General Information Unlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121 kDa; actual size ~150 kDa; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and ma SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Rat SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Antiserum Unpurified SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen The 15 AA rat NKCC21-P control peptide 100% conserved in mouse, 87% in human and rabbit NKCC2. No significant sequence homology is detected with NKCC1 or TSC. Antibody cross-reactivity in various species has not been studied SpCrossreactivity 15-aa peptide of Rat NKCC2/ Slc12a1 (gene accession # P55016); Designated (NKCC21-P or control peptide /blocking peptide) conjugated to KLH; Epitope location~ C-terminal, Cytoplasmic domain For research use only, not for diagnostic or therapeutic use.